With these prerequisites, high resolution and identification, I started the construction of a human myocardial database in 1990 still in the laboratory of Prof. Klose. In 1991 I was engaged at the German Heart Center in Berlin, where I built up the 2-DE laboratory and tried to identify in cooperation with Dr. Otto from Max Delbrück Center the first proteins of our 2-DE patterns (Jungblut et al., 1992a). This database was extended with identification data (Jungblut et al., 1994). At that time two problems limited the progress: The 2-DE system, was not commercially available and the sensitivity of identification was not sufficient to identify more than the most intensive spots of a gel. Therefore, Prof. Wittmann-Liebold and I founded a company, Wittmann Institute of Technology and Analysis of Biomolecules (WITA), in 1992. WITA sells equipment, service and know-how of the 2-DE technique developed by Klose. The method is exhaustively described with each detail, also for a further increase in gel size up to 30 x 40 cm, by Klose and Kobalz, 1995.
The sensitivity of identification was improved during the next years in the collaboration of WITA with the Protein chemistry group at the Max Delbrück Center. Whereas in the first years N-terminal sequencing and amino acid analysis were the most preferred methods for identification (Jungblut et al., 1992b), today internal sequencing and mass spectrometry are more effective (Thiede et al., Jungblut et al., Otto et al., Müller et al., 1996). We found that our data are in relatively good accordance with the data of Harefield heart database. After a careful check Dr. Müller created the heart high-performance 2-DE database update 1996. I hope this database will be a help for the project of protein analysis on the genomic level (Jungblut and Wittmann-Liebold, 1995) or proteome analysis (Kahn, 1995).
Nov 22nd, 1996, P. Jungblut
Since March 2007 the database is hosted at the Max Planck Institute for Infection Biology.
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Further we thank for financial support obtained by WITA.
We thank for contributions, help and fruitful discussions
|R. Benndorf||M. Dunn||C. Eckerskorn||E. Fleck|
|U. Kobalz||F. Lottspeich||A. Müller||U. Müller-Werdan|
|K.-P. Pleißner||J. Prehm||V. Regitz-Zagrosek||J. Salnikov|
|C. Scheler||J. Stahl||K. Werdan||E. Zeindl-Eberhart|
E.-C. Müller, A. Otto, P. Jungblut